|
KMID : 0880220180560090683
|
|
Journal of Microbiology
2018 Volume.56 No. 9 p.683 ~ p.689
|
|
|
Antiviral activity of Schizonepeta tenuifolia Briquet against noroviruses via induction of antiviral interferons
|
|
Ng Yee Ching
Kim Ye-Won
Lee Jeong-Su
Lee Sung-Joon
Song Moon-Jung
|
|
|
Abstract
|
|
|
|
Human noroviruses are the causative agents of non-bacterial gastroenteritis worldwide. The rapid onset and resolution of disease symptoms suggest that innate immune responses are critical for controlling norovirus infection; however, no effective antivirals are yet available. The present study was conducted to examine the antiviral activities of Schizonepeta tenuifolia Briquet extract (STE) against noroviruses. Treatment of human norovirus replicon-bearing HG23 cells with STE at 5 and 10 mg/ml concentrations resulted in the reduction in the viral RNA levels by 77.2% and 85.9%, respectively. STE had no cytotoxic effects on HG23 cells. Treatment of RAW 264.7 cells infected with murine norovirus 1 (MNV-1), a surrogate virus of human noroviruses, with STE at 10 and 20 μg/ml concentrations resulted in the reduction of viral replication by 58.5% and 84.9%, respectively. STE treatment induced the expression of mRNAs for type I and type II interferons in HG23 cells and upregulated the transcription of interferon-β in infected RAW 264.7 cells via increased phosphorylation of interferon regulatory factor 3, a critical transcription regulator for type I interferon production. These results suggest that STE inhibits norovirus replication through the induction of antiviral interferon production during virus replication and may serve as a candidate antiviral substance for treatment against noroviruses.
|
|
|
KEYWORD
|
|
|
antiviral activity, norovirus, Schizonepeta tenuifolia Briquet (ST), natural phytochemicals, interferons
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|